Journal: Nature Communications

Article Title: Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging

doi: 10.1038/s41467-025-64652-z

Figure Lengend Snippet: a Schematic of iNap1 based high-throughput screening for compounds. b The effects of 1419 compounds on iNap1 fluorescence ratio over iNapc fluorescence ratio. c The fluorescence ratio of cyto-iNap1 and cyto-iNapc with or without FA and shM1 overexpression in HAECs. n = 3 biologically independent samples, 101, 110, 111, 131, 112, 108, 120, and 117 cells for respective groups. Scale bar: 10 μm. d MTHFD1, p16, p21, and VCAM-1 protein levels in HAECs after shM1 overexpression. e p16, p21 and VCAM-1 protein levels with or without Ang II, FA and shM1 overexpression in HAECs. f Representative SA-β-Gal (upper) and EdU fluorescence (lower) staining images of HAECs with or without Ang II, FA and shM1 overexpression. Scale bar: 50 μm. Representative SA-β-Gal ( g ) and EdU fluorescence ( h ) staining images of old ECs with or without FA and shM1 overexpression. Scale bar: 50 μm. i p16, p21 and VCAM-1 protein levels in old ECs with or without FA and shM1 overexpression. j RT-PCR analysis of IL-1β, IL-6, IL-8 and CXCL1 mRNA levels in old ECs with or without FA and shM1 overexpression. n = 5 biologically independent samples. The fluorescence ratio of cyto-roGFP ( k ) and cyto-Hyper ( l ) with or without FA and shM1 overexpression in old ECs. n = 3 biologically independent samples, 122, 114, 117, 119, 117 and 118 cells for respective groups. Data are presented as mean values ± SEM. Statistical significance was assessed by One-way ANOVA followed by Tukey’s multiple comparisons test ( c , k , l ), Two-way ANOVA followed by Tukey’s multiple comparisons test ( j ).

Article Snippet: 1419 FDA-approved drugs were purchased form TargetMol Chemicals Inc. and 100 μM drugs were directly added into HAECs.

Techniques: High Throughput Screening Assay, Fluorescence, Over Expression, Staining, Reverse Transcription Polymerase Chain Reaction